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Fig. S11 Efficient knockdown activity of MO1/MO2-popdc2 morpholinos at 4 and 5 dpf. RT-PCR analysis of total RNA isolated of MO1/MO2-popdc2 and wild type embryos (WT) at 4 and 5 dpf. At both time points, MO1/MO2-popdc2 morphants display a significant decrease in popdc2 transcripts. These transcripts are aberrantly spliced and do not code for a functional protein as revealed by intron 1-specific PCR amplification. In each case expression analysis of β-actin served as a control.
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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.