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Fig. 7 Cardiac dysmorphogenesis in the popdc2 morphant. Zebrafish embryos were injected with 2 ng/embryo of (A,C,E) control morpholino (MO-CTR) or (B,D,F) MO1/MO2 popdc2 morpholinos. (A,B) Confocal analysis of 3 dpf transgenic Tg(cmlc2:GFP) hearts. The heart of the popdc2 morphants is dysmorphic and lacks trabeculation. (C,D) Anti-Alcam antibody staining reveals normal cell shape in the popdc2 morphants. (E,F) Anti-tropomyosin antibody staining shows a reduction in myofibrillar content in cardiac myocytes of the popdc2 morphants. Abbreviations: A, atrium; V, ventricle. Scale bar = 10 μm.
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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.