ZFIN Image: Liao et al., 2012, Fig. S1

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Figure Caption

Fig. S1 Gene trap lines appais22Gt and aplp2is23Gt contain Tol2 transposon integrations. A: SphI digested genomic DNA from wild-type (1), heterozygous appais22Gt/+ (2), and homozygous appais22Gt/is22Gt (3) adults, blotted and hybridized with an appa specific probe. The wild-type restriction fragment of <6.2 kb (arrow) is shifted to <7.2 kb (arrowhead) as expected due to the Tol2 integration in transgenic appais22Gt embryos. The faint higher band in lane 1 might be due to incomplete digestion or polymorphism in the appa gene in the WIK wild-type strain. The sample run in lane 2 is most likely DNA from a homozygous instead of heterozygous adult. B: SacI digested genomic DNA from wild-type (1), heterozygous aplp2is23Gt/+ (2), and homozygous aplp2is23Gt/is23Gt (3) adults, blotted and hybridized with an aplp2 specific probe. The wild-type restriction fragment of <12.1 kb in the aplp2 gene (arrow) is shifted to the expected size of <15.1 kb (arrowhead), due to the <3 kb Tol2 transposon integration, in the heterozygous aplp2is23Gt/+ adult. The homozygous aplp2is23Gt/is23Gt adult has one band at the expected size of <15.1 kb.

Acknowledgments

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