ZFIN Image: Cole et al., 2011, Fig. S1

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Figure Caption

Fig. S1 Donor somite transplants contribute only muscle to the host. (A) Fish transgenic for a ubiquitously expressed promoter (beta actin2), expressing mCherry (Tg(bact2:mCherry)^pc3), are crossed with fish transgenic for GFP driven by the alpha actin skeletal muscle specific promoter (Tg(acta1:GFP)^zf13). (B) The recipient host embryos are transgenic for BFP also expressed via the alpha actin skeletal muscle specific promoter (Tg(acta1:BFP)^pc5). (C) The donor somites from embryos doubly transgenic for Tg(bact2:mCherry^pc3) and Tg(acta1:GFP^zf13) were transplanted into a Tg(acta1:BFP^pc5) host. In each of 12 transplants performed in this way, co-expression of both green and red fluorescent protein was only ever observed, indicating that the donor somite somitic tissue only ever generated donor-derived muscle in the host. (D–G) Transplant of somite 10 using the method outlined in (C).

Acknowledgments

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