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Fig. S3 Effect of excessive RA treatment during 10–11 hpf on expressions of gata5 and gata6 in the RA-treated embryos at 14 hpf. All flat-mounted embryos are positioned anterior left and dorsal front. Embryos treated with vehicle DMSO (A, C) and 250 nM RA from 10–11 hpf were examined for expressions of gata5 (A, B) and gata6 (C, D) at 14 hpf, respectively. Expression of myoD in somites was used for staging and ntl expression was used for labeling embryonic axial mesoderm. The number shown in the lower left-hand corner of each panel is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed. The embryos treated with 250 nM RA from 10–11 hpf exhibited similar expression of gata5 but somehow increased expression of gata6 in the region between the anterior ends of their expression domains and the anterior end of ntl expression domain; however, they displayed significantly increased gata5 expression but greatly reduced gata6 expression in the region between the posterior ends of their expression domains and the anterior end of ntl expression domain, respectively.