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Fig. 1 Excessive RA inhibits primitive myelopoiesis in zebrafish embryos in a dose dependent manner.
All embryos are positioned anterior left and lateral front. Embryos were treated with vehicle DMSO (A, G), 6.25 nM (B, H), 12.5 nM (C, I), 25 nM (D, J) and 50 nM RA (E, K) respectively from 1–2-cell stage until 26 hpf or microinjected with cyp26a1-MO, cyp26b1-MO and cyp26c1-MO together at 1–2-cell stage (F, L). They were then examined for expressions of myeloid markers lcp1 (A–F) and mpx (G–L) at 26 hpf by whole mount in situ hybridization. The number shown in the lower left-hand corner of each panel is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed.