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Fig. 5
Whole-mount in situ hybridization with sonic hedgehog. Wild type (A) and dackel mutant embryo (B) at 28 hpf. Mutant dak embryos have reduced staining in the pectoral fin buds (arrowheads); no differences were detected in other regions that express this gene (data not shown). Wild type (C) and dak mutant embryo (D) at 32 hpf. In contrast to wild type, no shh expression above background is detectable in the pectoral fins of dak mutants at this age (arrowheads).
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