ZFIN Image: Bedell et al., 2012, Fig. S4

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Figure Caption

Fig. S4

Pronephric tubule and duct marker Tg(enpep:GFP) shows no changes in pronephric patterning in ponzr1 morphants. (A-P) Wild-yype Tg(enpep.4:GFP) embryos at 28 hpf (A, dorsal; B, lateral) and 48 hpf (I, dorsal; J, lateral) show fluorescence in the pronephric tubules and ducts. Mismatch MO-injected embryos show normal fluorescence patterns at 28 hpf (C, dorsal; D, lateral) and 48 hpf (K, dorsal; L, lateral). Embryos were injected at 1 ng (E,F) and 1.5 ng (G,H) of ponzr1 MO1 show no differences in the amount and localization of the fluorescence at 28 hpf (E,G, dorsal; F,H, lateral) and 48 hpf (M,O, dorsal; N,P, lateral). This experiment was conducted twice with at least 15 embryos in each group.

Acknowledgments

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