Fig. 5
Phenotypic analysis of zebrafish tcof1 MO-knockdown.
Lateral views (anterior to the left) of 15 hpf (A?B); 20 hpf (C?D); 25 hpf (E?F) embryos; and 5 dpf (G?J) larvae. Embryos were injected at the 1-cell stage with 1× Danieau (A, C, E, G and H) or in6:ex7-MO+ex7:in7-MO (B, D, F, I and J). At 20?25 hpf, the retina and midbrain-hindbrain regions showed a dark pigmentation (dotted lines), suggesting the presence of apoptotic cells. Abbreviations: ey, eye; he, heart edema; hv, hindbrain ventricle; MHB, midbrain-hindbrain border; sb, swim bladder; y, yolk. Scale bar: in A, 112.5 μm for A?B; in C, 160 μm for C?D, 260 μm for E?F, and 360 μm for G and I.