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Fig. S5
Characterization of linc-birc6 Expression in Zebrafish Embryos, Related to Figure 6 (A) In situ hybridization showing megamind expression in the brain of zebrafish embryos at 72 hpf. (B) Reduced megamind levels in embryos injected with the megamind splice-site MOs measured by qRT-PCR. (C) In situ hybridization of megamind expression in zebrafish embryos injected with the splice site MOs. (D) Embryo at 28 hpf that had been injected with the indicated reagents. Bottom panel shows RFP expression. Arrow points at defects in brain development.
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Reprinted from Cell, 147(7), Ulitsky, I., Shkumatava, A., Jan, C.H., Sive, H., and Bartel, D.P., Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution, 1537-1550, Copyright (2011) with permission from Elsevier. Full text @ Cell