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Fig. 4 Migration of the PLL primordium is altered in lbx2 morphants.
Migrating PLL atoh1a-expressing hair cells in control embryos (A, C, E) and lbx2 morphants (B, D, F) at 36 hpf (A, B), 40 hpf (C, D) and 48 hpf (E, F). (G) Analysis of the distance of PLL primordium migration in 48 hpf lbx2 morphants and controls. (H, I) TUNEL assay coupled with DAPI staining, indicating elevated cell death in the slowly migrating PLL primordium and deposited neuromasts of lbx2 morphants (I) compared with a similar region of the migrating PLL primordium in control embryos (H) at 36 hpf. Violet dots mark the migrating PLL primordium labeled with DAPI, stippled white lines indicate the zone of the PLL primordium and red dots indicate TUNEL positive cells.