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Fig. 6 Simultaneous overexpression of tfap2a and foxd3 induces ectopic neural crest. (A–D) Dorsal view (anterior to the left) showing expression of sox10 at 12 hpf in wild-type (WT) embryos (A), embryos injected with 150 pg of tfap2a mRNA (B), 15 pg of foxd3 mRNA (C) or 150 pg of tfap2a and 15 pg of foxd3 mRNAs (D). Arrowhead indicates ectopic sox10 expression in tfap2a + foxd3 mRNA injected embryos. (E–H) Gene expression in wild-type (WT) and tfap2a + foxd3 mRNA injected embryos. Lateral view (anterior to the left) of fgf8 (E) expression at 8 hpf and pea3 (F) expression at 10 hpf. Dorsal view (anterior to the top) of egr2b (r3, r5) and six3b (fb) at 12 hpf (G). Animal view (dorsal to the bottom) of hgg1, dlx3b, and shh (H) expression at 10 hpf. Abbreviations: fb, forebrain; ne, neural ectoderm; nne, non-neural ectoderm; pp, prechordal plate r3, rhombomere 3; r5, rhombomere 5.
Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.