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Fig. 3 tfap2a and foxd3 are expressed in complementary territories during gastrulation. (A–C) Lateral views of embryos showing expression of tfap2a in the non-neural ectoderm at shield and 75% and 90% epiboly. The dorsal (d), ventral (v), anterior (a) and posterior (p) sides are indicated. (D–I) Lateral (D–F, dorsal to the right) and dorsal (G–I, anterior side up) views of foxd3 expression in the organizer and at the dorsal margin at shield and 75% epiboly. By 90% epiboly, expression is restricted to axial mesoderm and lateral margin involuting cells. (J, K) Double in situ hybridization of tfap2a (blue) and foxd3 (red) at 75% epiboly showing the mutually exclusive, complementary expression pattern of the two factors in a lateral (J) and vegetal (K) view. (L) Digitization of tfap2a and foxd3 expression territories during gastrulation relative to the induction area of neural crest based on the corresponding in situ hybridization expression data at 75% epiboly.
Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.