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Fig. S3
lhx2/lhx9 morphant embryos show defect in thalamic neuron differentiation. A single in situ hybridization approach was used for analysis and all embryos were mounted laterally except in (I2, j2) showing cross-section of left hemispheres. Stages are indicated. In Lhx2/Lhx9-deficient embryos, lef1 expression in the thalamus (asterisk) is unaltered at 24 hpf but down-regulated at 3 dpf (a–d). Similarly, the Wnt target gene axin2 shows no alteration in Lhx2/Lhx9-deficient embryos at 20 hpf (e, f), however at 3 dfp an up-regulation can be detected in the mid-diencephalon (g, h). In control MO embryos, wnt1 is expressed at 48 hpf in the roof plate (RP) and lhx2/lhx9 morphant embryos display an expansion of the wnt1 expression domain into the thalamic territory (i–j2). In contrast tcf7l2 shows no alteration in the expression pattern at the same stage in the caudal forebrain. HyTh, hypothalamus; pTu, posterior tuberculum; RP, roof plate; Tec, tectum; Tel, telencephalon.