ZFIN Image: Neto et al., 2012, Fig. 4

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Figure Caption

Fig. 4

Osr mRNAs rescue tbx5a and pax2a expression in double morphants embryos. Whole-mount in situ hybridization using tbx5a and pax2a riboprobes on zebrafish embryos injected with osr1/osr2 morpholinos and osr1/osr1 splicing insensitive mRNA (MTosr1, MTosr2), as indicated. Panels show a dorsolateral view at 24 hpf. In each embryo, the limb bud is encircled in red and the pronephros is indicated with an arrowhead. (A) Control uninjected embryo. (B) Embryos co-injected with MOors1 and MOosr2. (C,D) Embryos co-injected with the two Osr morpholinos and the MTosr1 (C) or the MTosr2 (D) mRNA. Co-injection of either MTosr1 or MTosr2 mRNA rescues tbx5a and pax2a expression in limb buds and pronephros, respectively.

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Expression / Labeling details

Acknowledgments

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