ZFIN Image: Neto et al., 2012, Fig. 3

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Fig. 3

Osr genes are required for tbx5a expression during fin bud formation. Dorsal (A-P,U-X) and lateral (Q-T) views of tbx5a whole-mount in situ hybridization on zebrafish embryos injected with MOosr1, MOosr2 or a combination of both, as well as control embryos. Anterior is towards the left. (A-P) Osr MO-injected embryos reveal a smaller tbx5a expression domain in the pectoral fin field when compared with controls. Red arrowheads (A-L) or circles (M-P) mark tbx5a expression in the developing fins. (Q-T) At 24 hpf, the expression of tbx5a in the eye is not affected in morphant embryos. (U-X) tbx5a and pax2a double in situ hybridization. Red arrowheads and blue arrows indicate tbx5a and pax2a expression in the fin buds and anterior pronephros, respectively. The anterior pax2a expression domain disappears in MOosr1 and MOors1+MOosr2-injected embryos.

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Expression / Labeling details

Acknowledgments

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