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Fig. 5
Analysis of subcellular localization of zebrafish 20beta-HSD type 2 showed that the enzyme is localized in the endoplasmic reticulum. Pearson’s correlation coefficient for colocalization of 20beta-HSD type 2 and the endoplasmic reticulum marker is 0.84. The panels depict detection of recombinant 20beta-HSD type 2 with anti-myc FITC-labeled antibody (A), counterstaining of endoplasmic reticulum with DsRed (B), and nuclei with Hoechst 33342 dye (C), respectively, and the overlay of fluorescence patterns (D). Magnification: 40x.
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Reprinted from Molecular and Cellular Endocrinology, 349(2), Tokarz, J., Mindnich, R., Norton, W., Möller, G., Hrabé de Angelis, M., and Adamski, J., Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2, 202-13, Copyright (2012) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.