ZFIN Image: Goodale et al., 2012, Fig. 6

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Figure Caption

Fig. 6

CYP1A protein expression patterns are ligand- and AHR isoform-dependent.

CYP1A expression at 120 hpf in (A) ahr2⁺ and (B) ahr2^hu3335 larvae following exposure to 1 nM TCDD from 6–24 hpf. C) Leflunomide-induced CYP1A expression at 72 hpf in wild-type and (D) ahr2^hu3335 mutants. E) Leflunomide-induced CYP1A expression in AHR1B-morphant ahr2^hu3335 larvae and F) ahr2^hu3335 larvae co-injected with AHR1A and AHR1B morpholinos. (G) DMSO control. TCDD-exposed embryos were IHC processed side-by-side and imaged at 120 hpf using the same exposure settings and a single focal plane. Leflunomide-exposed embryos and DMSO control were processed side-by-side and imaged at 72 hpf using the same exposure times; images were created from a z-stack of 10 15.4 uM slices centered on the liver.

Acknowledgments

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