ZFIN Image: Powell et al., 2011, Fig. 6

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Figure Caption

Fig. 6

Interaction between jamb and jamc is necessary and required in trans for myocyte fusion.

(A–D) Fluorescent dextran-labelled cells (magenta) from jamb^HU3319 and jamc^sa0037 donors can form multinucleate fibres with wild-type (A, B), jamc^sa0037 (C), or jamb^HU3319 (D) host cells, respectively. (E, F) Transplanted cells from doubly-deficient donors fail to fuse with wild-type host cells, suggesting both proteins are required and interact in trans. Confocal microscopy images from 48 h. p. f. embryos; anterior left. Schematics illustrate potential binding of Jamb and Jamc between donor (magenta) and host (green) cells in each experiment. bMO, cMO indicate jamb or jamc translation-blocking morpholino-injected donor embryos. Dotted lines indicate the position of myotome boundaries; arrowheads indicate nuclei within labelled fibres. Nuclei stained with DAPI (green). Scale bars represent 20 μm.

Acknowledgments

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