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Fig. S6 RA pathway components are highly expressed during regenerative outgrowth. Whole-mount in situ hybridization at 48 and 72 hpa (A) and in situ hybridization on longitudinal sections at 72 hpa (B) reveal expression of aldh1a2, rdh10a and rarga in the blastema. rarga and rdh10a transcripts can be detected in the whole blastema at 72 hpa, whereas aldh1a2 expression is more restricted to distal blastema cells. Dashed lines indicate amputation plane. Scale bars: 200 μm in A; 100 μm in B.
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