ZFIN Image: Carten et al., 2011, Fig. 7

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Figure Caption

Fig. 7 Fat-free (ffr) larvae display abnormal bile accumulation upon BODIPY-FL C₅ feeding. A. Wildtype siblings (controls) from a cross of ffr +/- larvae (6dpf) exhibit normal hepatic accumulation of BODIPY-FL C₅ in lipid droplets (filled arrowhead) and hepatic ducts (arrow). B. Hepatic ducts in ffr -/- larvae leak or abnormally accumulate BODIPY-FL C₅, consistent with the hepatobiliary structural malformations previously identified in these mutants. C. Analog labeling also reveals ffr -/- hepatocytes are unable to secrete bile as indicated by the appearance of cholestatic livers. D. A higher magnification of C. reveals abnormal accumulation of bile in a single ffr -/- hepatocyte. Scale bars = 10μm. (n = 3 feeds; 10 larvae per feed).

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Acknowledgments

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Reprinted from Developmental Biology, 360(2), Carten, J.D., Bradford, M.K., and Farber, S., Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish, 276-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.