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Fig. 2 pax9 CNEs and modified BAC. (A) UCSC browser snapshot showing synteny around pax9 and the functional CNEs Px2, Px4 and Px7. (B) Whole mount RNA in situ hybridization showing expression of pax9 in branchial arch and dorsal sclerotome at 36 hpf and (C) 48 hpf. (D) Functional CNE Px2 drives expression of EGFP in sclerotome/myotome (SC/MY) as well as dorsal sclerotome at 36 hpf. Enhancers Px4 (E) and Px7 (F) drive expression in the sclerotome and myotome at 36 hpf and 48 hpf respectively. (G) Modified BAC showing expression in the dorsal sclerotome at 36 hpf. BA - branchial arch. DS - dorsal sclerotome