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Fig. 2 abca12 and snap29 gene expression in normal zebrafish. (A–C) Zebrafish embryos were collected at 0 and 6 hpf and 1–8 dpf, and total RNA was isolated and cDNA prepared. The abca12 (A) and snap29 (C) mRNA expression levels were measured by RT-PCR and standardized against the mRNA expression level of the β-actin gene. (B) Whole-mount in situ hybridization of embryos at different stages of early development for abca12 expression; gastrula period (left panel), 24 hpf (right panel).
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Full text @ Dis. Model. Mech.