ZFIN Image: Nguyen et al., 2011, Fig. 6

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Figure Caption

Fig. 6 Kras^V12-induced p53-dependent senescence in the pre-neoplastic liver. (A) Oncogenic Kras^V12-induced senescence at an early stage of liver tumor development. SA-βgal staining was performed on liver cryosections from 3- and 9-mpf kras^V12 transgenic and WT fish (tp53^+/+). (B) Western blots of total proteins from liver hyperplasia (H), carcinoma (C) and age-matched WT liver to evaluate the levels of tumor suppressor p53 together with its target, p21 Waf1/Cip1, and its regulator, phospho-MDM2, during tumorigenesis. β-actin, internal control for equal loading. (C) Acceleration of liver tumor onset in homozygous tp53^M214K mutant transgenic fish. Percentages of tumors observed from kras^V12 transgenic fish with different p53 backgrounds at different time points are shown. (D) Suppression of senescence induced by oncogenic kras^V12 in tp53^-/- background. SA-βgal staining was performed with four biological replicates in each group. Scale bars: 50 μm.

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Acknowledgments

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