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Fig. 3 qilin is expressed maternally and ubiquitously.
(A–F) In situ hybridization for qilin on embryos at the 8- cell stage (A), the shield stage (B), the 3–5 somites stage (C), and the 24 hours-post-fertilization (hpf) stage (E). D and F are sense controls at the 3–5 somites stage (D) and the 24 hpf stage (F). (G) qilin transcript shown by RT-PCR from 16 cell (Lane 1) and 2 dpf (Lane 2) wild type embryos. Lane 3 and 4 are elf1a loading controls for 16-cell (Lane 3) and 2 dpf (Lane 4) samples with matching dilutions of cDNA used in PCR reactions.
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