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Fig. 4
Immunohistochemistry at 72 hpf shows retraction of epitopes with detaching fibers, demonstrating that failure occurs within the extracellular matrix rather than at the sarcolemma. (A) caf, anti-dystrophin. (B) Wild-type anti-dystrophin. (C) caf, anti-β-dystroglycan. (D) Wild-type anti-β-dystroglycan. (E) caf, anti-laminin 1. (F) Wild-type anti-laminin 1. (i) Flourescence, (ii) DIC, and (iii) merge. Arrowheads indicate retraction of epitopes with individual fibers. Images show lateral views, anterior to the left.
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