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Fig. S10
Effect of cyclopamine treatment on early NCC migration. (A) Adding cyclopamine at 4 hpf causes no defect in CNCC migration at 19 hpf shown by Sox10:eGFP expression. (B) At 30 hpf, the embryos show morphological signs of Shh pathway inhibition, but CNCCs have still migrated into the head, showing that Shh signaling is not important for initial migration of cells but is critical for patterning.
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