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Fig. S8
Effects of reduction of nipbls on endodermal and Nodal-signaling genes. (A) Expression of endodermal genes (sox17, foxa2, and gata5) was examined by ISH at 8.5 hpf. Expression of all three was downregulated in endoderm (arrows) but not in dorsal forerunner cells (sox17; arrowheads) or axial mesoderm (foxa2; brackets). gata5 expression was mainly reduced dorsally (arrow). (B) In contrast, no changes were observed in expression of the Nodal-related gene, cyc, a Nodal receptor co-factor, oep, or a mesodermal Nodal target, ntl, in nipbla/b-morphants compared with controls at 6.5 hpf. Lateral views with dorsal to the right in all cases except gata5, which is shown in dorsal view, anterior to the left. Scale bars: 50 μm. (C) Expression of endodermal and nonendodermal genes were examined by Q-PCR at 7 hpf in controls (black) and nipbla/b-morphants (red). Expression levels are normalized to that of ef-1a and shown relative to values in control embryos. p values were calculated by paired t test, using ΔCt values (n = 4, mean ± S.E.M., * p<0.002 and others are p>0.1).