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Fig. S14 Hh signaling, basal Gli activity and RA signaling cooperate to promote motoneuron progenitor patterning. (A-O) Embryos were treated with (A,D,G,J,M) DMSO, (B,E,H,K,N) 100 μM cyclopamine, or (C,F,I,L,O) 100 μM SANT75, in combination with (D-F) 10 μM DEAB, (G-I) a gli1 MO, (J-L) genetic loss of gli1 function (gli1ts269 mutant), or (M-O) a gli2b MO. The embryos were then fixed at the bud stage and stained for olig2 transcripts to mark motoneuron progenitors. Scale bar: 100 μm.
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