ZFIN Image: Mich et al., 2011, Fig. 1

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Figure Caption

Fig. 1 MZsmo zebrafish embryos have patterning defects and lack detectable Hh signaling. (A-C) Wild-type, smo and MZsmo embryos (24 hpf). (D-F) Maternal smo mRNA is present in zygotic smo mutants but depleted in MZsmo embryos. Homozygous smo^hi1640 embryos could be distinguished from siblings at the shield and bud stages but not at the two-cell stage. Two-cell and shield embryos are viewed laterally; bud-stage embryos are flat-mounted and viewed dorsally, anterior to left. (G-I) Maternal smo-dependent nkx2.2a expression in the zona limitans intrathalamica (arrowheads). (J-O) Wild-type, smo and MZsmo embryos (10 hpf) were either uninjected or injected with shha mRNA and stained for nkx2.2a (primary micrographs) or ptc1 (insets) transcripts. Images are lateral views of the anterior neural plate, anterior to the left. (P) Wild-type and MZsmo embryos were treated as in J-O and analyzed by qRT-PCR. Transcript levels are normalized to the wild-type control; error bars represent s.e.m. Scale bars: 200 μm in A-I; 100 μm in J-O.

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