ZFIN Image: Alexander et al., 2011, Fig. 3

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Figure Caption

Fig. 3

BMP signaling is required for ventral cell specification in the arches. (A-P) Whole-mount in situ hybridization to detect expression of genes involved in DV arch patterning, lateral views, anterior towards the left in controls (A,C,E,G,I,K,M,O) and Tg(hsp70I:dnBmpra1-GFP) transgenics heat shocked at 16-18 hpf (B,D,F,H,J,L,N,P). dlx3b (A,B), dlx6a (C,D) and hand2 (E,F) expression is reduced at 28 hpf. nkx3.2 (G,H) expression is reduced and jag1b expression (I,J) expands ventrally at 36 hpf (arrows). msxe expression is lost at 30 hpf (K,L). Arches 1 and 2 are numbered. (M-P) Two-color fluorescent in situs to detect dlx3b (M,N, red) or msxe (O,P, red) simultaneously with hand2 (green). Scale bar: 100 μm.

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