ZFIN Image: Muto et al., 2011, Fig. 1

Image

Figure Caption

Fig. 1 Zebrafish nipbl genes.

(A) Domain structure of Nipbla and Nipblb proteins. N- and C-terminal conserved regions are shown in gray; predicted domains and motifs as colored boxes. Pink lines indicate positions used for antisense RNA probes. (B) Comparison of Nipbl orthologs among species. Numbers indicate amino acid identity to human NIPBL, as determined by Clustal W analysis (http://clustalw.ddbj.nig.ac.jp/top-j.htm l). (C) Phylogenic tree of Nipbl proteins, constructed by the NJ method (http://www.ddbj.nig.ac.jp/), using full-length amino acid sequences from the Ensembl database. (D) Expression of nipbla and nipblb mRNAs at 9 hpf was analyzed by Northern blotting (10 µg total RNA per lane) using DIG-labeled antisense RNA probes. Two different probes for each gene (see panel A) were used. (E) Expression patterns analyzed by ISH with nipbla (upper) and nipblb (lower) AS1 probes at indicated stages. Views are lateral, with anterior to the top (2.75–15 hpf) and left (18–35 hpf). Scale bar: 100 μm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.