Fig. 9

Fig. 9 Myofibril defects are rescued with exogenous rbfox2. (A–C) Anti-MHC immunohistochemistry at 48 hpf reveals that slow muscle fiber thick filament morphology in rbfox2 mRNA-injected double morphant embryos closely resembles wild-type morphology and is substantially improved compared to that in embryos injected with rbfox1l/rbfox2 MOs alone, although some wavy fibers persist. (D–I) Similarly, thin filament structure detected using Alexa-Fluor 488-conjugated phalloidin appears elongated and highly organized in both deeper fast muscle and more superficial slow muscle layers of rbfox2 mRNA-injected double morphants when compared to mock-injected wildtype controls and embryos injected with rbfox1l/rbfox2 MOs alone. Scale bar = 50 μm.