Fig. 3
Swap70 is localized to nuclear and cytoplasmic compartments. Cherry-Swap70 was transiently expressed by crossing Tg(hsp70l:Gal4vp16);Tg(olig2:EGFP) fish to Tg(UAS:Cherry-Swap70) and performing heat shock induction. Two examples (A, A2 and B, B2) showing nuclear localization of Cherry-Swap70. (A, A2) Z-stack images were merged in single plane. An olig2-EGFP- cell (arrow) next to olig2-EGFP+ cell (arrowhead) in anterior–posterior position had nuclear localized Cherry-Swap70. The inset is an X-Z projection of the image. (B, B2) Single plane image showing nuclear localization of Cherry-Swap70 in an olig2:EGFP+ precursor cell (arrow). Nuclei are labeled with DAPI (blue).
Reprinted from Molecular and cellular neurosciences, 48(3), Takada, N., and Appel, B., swap70 Promotes neural precursor cell cycle exit and oligodendrocyte formation, 225-35, Copyright (2011) with permission from Elsevier. Full text @ Mol. Cell Neurosci.