|
Fig. S2
PRMT5 and CARM1 regulate specifically myogenic factors expression at 14-somite stage (14 ss). Whole-mount in situ hybridization of embryos injected at one-cell stage with the indicated morpholino (Mo). Experimentrs were done twice with n = 20 for each condition. Embryos were collected at 14 ss and were analyzed for myogenic factors expression by (A) in situ hybridization, lateral view, anterior to the left or by (B) real time PCR with standard deviations relative to a control morpholino. Q-PCR procedures are detailed in the methods section. (A) Asterisk, anterior expression of myf5 in CARM1 morphant; arrow, down regulation of myogenin expression in the posterior somites in PRMT5 morphant. (B) Error bars represent standard deviations. *, P<0.01; **, P<0.001; ***, P<0.0001; ns, not statistically significant.