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Fig. S12 Stab lesion activates proneural and neurogenic gene transcription specifically in the VZ. (A) The expression of the proneural gene ascl1 is strongly upregulated in the dorsal and lateral part of the VZ of the lesioned dorsal telencephalon 3 dpl, compared with the control hemisphere (arrows). In situ hybridisation in 14 µm cryosections shows that ascl1 is only weakly expressed in the lateral part of the dorsal VZ in the control hemisphere (insets). Expression along the medial ventricle is not obviously altered in the lesioned side. (B) By 3 dpl, the expression of the neurogenic gene deltaD is strongly upregulated in the dorsal and dorsomedial VZ (dashed line) of lesioned hemispheres, as detected by in situ hybridisation. Scale bars: 200 μm in A, 100 μm in insets and in B. Dashed circles represent the lesion canal.