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Fig. S9 Mnx1 MO1 but not Mnx1 MO2 disrupts Tg(mnx1:GFP) expression. (A-P) Confocal images of Tg(mnx1:GFP) embryos at (A-D) 16 hpf, (E-H) 18 hpf and (I-P) 24 hpf. (A-L) Immunolabeling with GFP and myosin antibodies. Endodermal Tg(mnx1:GFP) cells (green) are observed in the midline adjacent to somites (S) (blue). Dorsally localized GFP-expressing cells anterior to the somites are motoneurons (arrowheads). (M-P) Higher magnifications. (I-L) Colabeling of GFP cells with insulin antibody (red).
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