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Fig. S7 Altered balance of hormone producing cells is maintained in 60 hpf mnx1 morphants. (A-P) Confocal images of primary pancreatic islet from 60 hpf wild-type (AB) embryos. Immunolabeling for (A-D) insulin, (E-H) glucagon, (I-L) somatostatin and (M-P) colocalization of all three hormones. Note disorganized primary islets in mnx1 morphants (O,P). (Q) Mean (± s.d.) number of endocrine cells. *, P<0.001 (t-test, two-tailed distribution, Bonferroni correction). Results are from a minimum of 15 embryos per group.
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