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Fig. S5 Tg(neurod:EGFP) is an endocrine progenitor marker. (A-C) Confocal image of 30 hpf dorsal pancreas. (A) Immunostaining to detect Islet1 (red) and (B) GFP (green); (C) colocalization of Islet1 and GFP. (D-L) Confocal images of 72 hpf embryos showing primary islets. Immunostaining for (D) insulin, (G) glucagon, (J) somatostatin, and (E,H,K) GFP. (F,I,L) Colocalization of hormones and GFP. Anterior to left.
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