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Fig. S2
Potential target genes of proneural factors behave identically after mis-expression of Neurog1 and Neurod4. (A-J2) Whole-mount in situ hybridisation against neurog1 (A-B2), neurod4 (C-D2), deltaA (E-G2) and robo2 (H-J2) in wild-type (A,C,E,H) and hsp70:myc-neurog1 (D,D2,F,F2,I,I2) and hsp70:myc-neurod4 (B,B2,G,G2,J,J2) injected embryos at 10 hpf; injected embryos were heat-shocked at 7 hpf for 1 hour and fixed at 10 hpf. Heat-shocked embryos display ectopic expression of deltaA (E-G) but not of robo2 (H-J). In addition, mis-expression of Neurog1 is able to activate neurod4 expression. However, mis-expression of Neurod4 is not able to activate ngn1 expression. Confocal sections of the neural plate at 10 hpf show that mosaic mis-expression of a Myc-tagged form of Neurog1 or Neurod4 (in green and marked with an asterisk) induces deltaA (in red in F2,G2) in a cell-autonomous manner but not robo2 (I2,J2). However, whereas Myc-tagged Neurog1 induces cell-autonomous expression of neurod4 (in red in D2), Myc-tagged Neurod4 cannot induce neurog1 under similar conditions (Bý). Embryos are viewed dorsally with anterior up. Scale bars: in A, 100 μM for A-J; in B2, 100 μM for B2,D2,F2,G2,I2,J2.