|
Fig. S1
GFP expression from Tg(8.4neurog1:gfp) acts as a short term lineage label for endogenous neurog1-expressing cells. (A-C) Whole-mount in situ hybridisation against neurog1 in wild-type (A) or gfp from the Tg(8.4neurog1:GFP) transgene in wild-type (B) and neurog1 mutant embryos (C) at 10 hpf. Embryos are viewed dorsally with anterior down. At 10 hpf, neurog1 is expressed at the anterior border of the neural plate (A). In neurog1 mutant embryos, gfp+ cells are not lost in this territory (B versus C). Scale bar: 100 μM for A-C. (D-G2) Confocal sections of in situ hybridisation and immunolabelling against neurog1 and GFP in wild-type olfactory placodes in Tg(8.4neurog1:GFP) embryos at 14 (D,D2), 18 (E,E2), 20 (F,F2) and 22 (G,G2) hpf. neurog1 expression disappears between 20 and 22 hpf. Placodes are oriented with anterior up and lateral to the right. Dashed white line indicates the boundary of the telencephalon and the olfactory placode (E-G2). Scale bars: 10 μM.