ZFIN Image: de Borsetti et al., 2011, Fig. 4

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Figure Caption

Fig. 4 Production of melatonin is delayed under DD conditions and greatly reduced under LL conditions. (A) Zebrafish embryos were raised in 14/10 light/dark (LD), constant darkness (DD), or constant light (LL) conditions beginning at 5 min post-fertilization, and harvested for quantitation of melatonin by ELISA. (B) A 0.10 pg/embryo peak of melatonin production is detected in LD embryos at 45 hpf, in contrast to embryos under DD conditions, which reach 0.10 pg/embryo 10 h later. Melatonin concentration remains below 0.01 pg/embryo at all times in LL embryos. Graph shows the average of 3 independent experiments. (C–E) In situ hybridization for melatonin receptor 1ba (mtnr 1ba) and (F–H) melatonin receptor 1bb (mtnr1bb). Expression of mtnr1ba and mtnr1bb is found throughout the brain from 24 to 48 hpf. (I–K) In situ hybridization for cxcr4b reveals that habenular cells (black arrows) are present in the developing brain where mtnr1ba and mtnr1bb are expressed. Samples are siblings of the embryos in (C–H), photographed at the same focal plane. All views are dorsal. Scale bar = 50 μm.

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Reprinted from Developmental Biology, 358(1), de Borsetti, N.H., Dean, B.J., Bain, E.J., Clanton, J.A., Taylor, R.W., and Gamse, J.T., Light and melatonin schedule neuronal differentiation in the habenular nuclei, 251-61, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.