Fig. S1

Fig. S1 Expression of miR-206 in zebrafish development. In situ RNA hybridization for miR-206. Dorsal (A) or lateral (E) flatmounts, lateral (B, C, D, D′′), dorsal (C′′) or ventral (D′) wholemounts, anterior to top (A) or left (B–E). A. Expression is detected late in adaxial slow fibre differentiation and early in fast myogenesis. B. At 24 hpf, expression is restricted to the differentiated somitic muscle. C. By 48 hpf, expression is abundant in somitic fast muscle (arrowheads). A transverse section at the level indicated by the line in C, shows signal in somitic fast muscle (C′, black arrowheads). Notochord signal (red arrow) is detected in some embryos. Pectoral fin muscle expression is also detected (C′′, white arrowheads). D. At 72 hpf, the forming cranial muscles also contain miR-206. E. miR-206 expression is reduced in somitic muscle of myod^fh261 mutant and further reduced after cyclopamine treatment, indicating that miR-206 is expressed in slow muscle fibres. Bars = 100 μm.