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Fig. S5 npnt mRNA rescues the MO2 phenotype on a cellular and molecular level. (A) Confocal sections of whole-mount Alcam-stained (red: myocardium and differentiated endocardial cells) control, MO2-injected and MO2 + npnt mRNA-injected embryos from transgenic Tg(kdrl:EGFP)s843 zebrafish at 52 hpf. Note that injection of npnt mRNA rescued the MO2-mediated increase in Alcam-positive endocardial cells. Arrowheads indicate the AV boundary. (B) Quantitative analysis. MO2 + npnt mRNA-injected embryos were analyzed from morphologically rescued embryos. Mean ± s.e.m. (C) Representative images of hearts from control, MO2-injected and MO2 + npnt mRNA-injected embryos after whole-mount in situ hybridization with probes against bmp4 and cspg2 at 52 hpf. Note that injection of npnt mRNA rescued the MO2-mediated expansion of these genes. Brackets indicate the AV boundary. SAV, superior AV; IAV, inferior AV; A, atrium; V, ventricle. Scale bars: 50 μm.