ZFIN Image: Lu et al., 2011, Fig. S1

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Figure Caption

Fig. S1 Analysis of spatial localization of mRNAs coding for 23 zebrafish Wnt genes by whole mount in situ hybridization to identify those present in cortical cytoplasm of two-cell stage embryos. In situ hybridization was performed on wild-type embryos at the two-cell stage with specific antisense RNA probes corresponding to the different Wnts. The labeling reaction was performed for 24 h (10 times longer than usual) to detect possible low amounts of mRNA in the cortical cytoplasm. Due to strong overstaining, nonspecific labeling is observed in blastomeres for all Wnt probes. Only Wnt8a displays accumulation of mRNA in the cortical cytoplasm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA