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Fig. 7 Implantation of an Fgf4-soaked bead (A,C,E,G,I,K) or a PBS-soaked control bead (L) into dak- fin buds. (B,D,F,H,J,M) Contralateral fin bud of the same embryo not treated with Ffg4- or PBS-bead. Marker gene expression is shown at 60 h. (A,B) syu, (C,D) hoxa13, (E,F,L,M) hoxd11, (G,H) dlx2, (I,J) fgf4. Anterior is towards the left and distal is towards the top. (K) en1. Distal view, anterior is towards the top. All Fgf4 bead-treated buds are larger than the untreated buds on the contralateral side. All mesenchymal markers tested are activated. In the apical ectoderm only early markers are activated (dlx2, bmp2; see text) whereas late markers are not activated (fgf4, fgf8; see text). Note that en1 is activated. PBS-bead implantation does not lead to marker gene activation of control buds.