ZFIN Image: Fujimoto et al., 2011, Fig. 3

Image

Figure Caption

Fig. 3 Time course of Gal80-mediated inhibition of Gal4. Confocal images of live embryo green fluorescent protein (GFP) expression. Transgenic fish carrying Tg(hs:Gal80); Tg(otpb.A:Gal4); Tg(UAS:GFP) were imaged before a 452 heat-shock, and then at defined time intervals following heat-shock. The same embryo is displayed in each respective set of panels (A–A′′ ′′ or B–B′′ ′′). Arrow points to the inhibition of Gal4-dependent expression in (A′); arrowhead points to the return of GFP expression in (A′′ ′′) 24 hr following the end of heat-shock. Images are maximum projections of 10 z-slices. Ventral views, rostral to the top, identical confocal settings for imaging both embryos. Scale bar = 50 μm; “h”, GFP expression in the heart from the transgenesis marker.

Acknowledgments

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