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Fig. 8
Foxo3b interacts with β-catenin1/2 in 293T cell.
(A) Foxo3b inhibited β-catenin/T cell factor activity in 293T cell line. 293T cells were transfected with 3xTOPFlash, and the plasmids as indicated, together with pTK-renilla as an internal control; luciferase activity was measured after 24h. Date presented were the average (±SEM) of three independent experiments, performed in triplicate. “**” indicates p<0.01. (B) Foxo3b co-localized with β-catenin1/2. HeLa cells were transfected with GFP-β-catenin1 (B1-B3) or GFP-β-catenin2 (B4–B6), together with RFP-foxo3b. Transfected cells were then observed using fluorescent microscopy after 24h. (C) Foxo3b interacted with β-catenin1/2. 293T cells were transfected with the indicated expressing plasmids. HA-foxo3b was immunoprecipitated, and binding of Flag-β-catenin1 (C1) or Flag-β-catenin2 (C2) was analyzed by immunoblotting.