ZFIN Image: Eames et al., 2011, Fig. 3

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Figure Caption

Fig. 3

Wild-type fam20b expression rescues the fam20b^b1127 mutant phenotype.

A–E, Flat-mounted, dissected pharyngeal skeletons of Alcian blue/Alizarin red-stained 6 dpf larvae. Both sides of the pharyngeal skeletons are shown because the mosaic nature of the rescue experiment produced asymmetric phenotypes in injected larvae. Compared to uninjected wild-type (A) and mutant (B) controls, fam20b^b1127 mutant larvae that were injected with wild-type fam20b cDNA under the control of beta-actin2 promoter (C) showed skeletal elements with rescued cartilage matrix staining by Alcian blue and decreased bone matrix staining (asterisk) by Alizarin red. D, Higher magnification of boxed region in C illustrates patches of light Alcian blue staining surrounded by heavy Alizarin red staining (arrow, dashed lines) adjacent to patches of dark Alcian blue staining surrounded by light Alizarin red staining. E, fam20b^b1127 mutants embryos similarly injected with fam20b^b1127 did not rescue the mutant skeletal phenotype. Abbreviations: UIC = uninjected control.

Acknowledgments

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