Fig. S2
Adult lef1nl2 mutants show defects in fin and tooth formation. (A,B) Bright-field images of adult wild-type sibling (A) and lef1nl2 mutant (B) fish. Note that the lef1nl2 mutant lacks a caudal fin. (C-D′′) Alizarin Red staining in calcified bone in wild-type (C) and lef1nl2 mutant (D) fish. Lepidotrichia in both paired pectoral fins (arrows) and the caudal fin (arrowheads) are formed in the wild-type fish (C-C′′). By contrast, these structures are stunted in the lef1nl2 mutant (D-D′′). (E,F) Alizarin Red staining of the pharyngeal teeth and bone of the jaw and Alcian Blue staining of cartilage in the gill rakers. (E) Wild-type jaw with several pharyngeal teeth (black brackets). (F) By contrast, the lef1nl2 mutant jaw contains only one tooth (black arrow). Gill rakers appear normal in both wild-type and lef1nl2 mutant fish.